Extraction, characterization and comparison the components of Eruca vesicaria L and Eruca sativa Mill and study their  biological activity

Ghassan Radam Idan AL-Taie; Ahmed A. Ismail; Mostefa Khalid Mohammed; Ziad T I Alkyar; Nagham Thayer Salman

Authors

Ghassan Radam Idan AL-Taie Department of Biology, College of Education for Pure Science, University of Diyala, Diyala, Iraq.
Ahmed A. Ismail
Mostefa Khalid Mohammed
Ziad T I Alkyar
Nagham Thayer Salman

Keywords:

Eruca vesicaria Eruca sativa Phytochemical

Abstract

In this work high performance liquid chromatography (HPLC) was used to isolate and study the components of plants Eruca sativa and Eruca vesicaria, both are belonging to the family Brassicaceae. The extractions have done using Soxhlet apparatus. However, the result shows that there are some different in the compounds for each. The Eruca extracts are a powerful antioxidant agent and the analysis of both plant samples using the HPLC chromatogram with standard sample shows that the extracts of both plants contains an antioxidant compounds. The antibacterial activities of the extracts were tested against Escherichia coli, Staphylococcus aureus and the activity was good towards the selected bacteria. In addition, the extracts show a good antifungal activity towards candida albicans).

References

Ameen, O. M., Olatunji, G. A., Usman, L. A., Adebayo, M. A., Zubair, M. F., Opabola, T. O., & Agboola, D. O. (2015). The phytochemical screening and toxicity of the stem extracts of Dracaena manni (BAKER). Int J Chem Sci, 7, 17–25.

Bauer, A. W., PERRY, D. M., & KIRBY, W. M. M. (1959). Single-disk antibiotic-sensitivity testing of staphylococci: An analysis of technique and results. AMA Archives of Internal Medicine, 104(2), 208–216.

Bell, L., Oruna-Concha, M. J., & Wagstaff, C. (2015). Identification and quantification of glucosinolate and flavonol compounds in rocket salad (Eruca sativa, Eruca vesicaria and Diplotaxis tenuifolia) by LC–MS: Highlighting the potential for improving nutritional value of rocket crops. Food Chemistry, 172, 852–861.

Bell, L., & Wagstaff, C. (2014). Glucosinolates, myrosinase hydrolysis products, and flavonols found in rocket (Eruca sativa and Diplotaxis tenuifolia). Journal of Agricultural and Food Chemistry, 62(20), 4481–4492.

Bouacida, S., Koubaier, H. B. H., Snoussi, A., Fauconnier, M.-L., & Bouzouita, N. (2016). Glucosinolate profiles by HPLC-DAD, phenolic compositions and antioxidant activity of Eruca vesicaria longirostris: Impact of plant part and origin. Mediterranean Journal of Chemistry, 5(5).

Ehtaiwwesh, A. F., & Qarimidah, F. (2021). Aqueous Extract of (Eruca sativa Mill) as Growth Stimulant in Enhancing Growth and Yield of Faba Bean (Vicia faba L). Al-Mukhtar Journal of Sciences, 36(1), 57–66.

Jaafar, N. S., & Jaafar, I. S. (2019). Eruca sativa Linn.: Pharmacognostical and pharmacological properties and pharmaceutical prepara-tions. Asian J Pharm Clin Res, 12(3), 39–45.

Khan, A. U., Ullah, F., Khan, N., Mehmood, S., Fahad, S., Datta, R., Irshad, I., Danish, S., Saud, S., & Alaraidh, I. A. (2020). Production of organic fertilizers from rocket seed (Eruca sativa L.), chicken peat and Moringa oleifera leaves for growing linseed under water deficit stress. Sustainability, 13(1), 59.

Koubaa, M., Driss, D., Bouaziz, F., Ghorbel, R. E., & Chaabouni, S. E. (2015). Antioxidant and antimicrobial activities of solvent extract obtained from rocket (Eruca sativa L.) flowers. Free Radicals and Antioxidants, 5(1), 29–34.

Michael, H. N., Shafik, R. E., & Rasmy, G. E. (2011). Studies on the chemical constituents of fresh leaf of Eruca sativa extract and its biological activity as anticancer agent in vitro. J. Med. Plants Res, 5(7), 1184–1191.

Pasini, F., Verardo, V., Cerretani, L., Caboni, M. F., & D’Antuono, L. F. (2011). Rocket salad (Diplotaxis and Eruca spp.) sensory analysis and relation with glucosinolate and phenolic content. Journal of the Science of Food and Agriculture, 91(15), 2858–2864.

Pi?lewska-Bednarek, M., Nakano, R. T., Hiruma, K., Pastorczyk, M., Sanchez-Vallet, A., Singkaravanit-Ogawa, S., Ciesio?ka, D., Takano, Y., Molina, A., & Schulze-Lefert, P. (2018). Glutathione transferase U13 functions in pathogen-triggered glucosinolate metabolism. Plant Physiology, 176(1), 538–551.

Taffner, J., Cernava, T., Erlacher, A., & Berg, G. (2019). Novel insights into plant-associated archaea and their functioning in arugula (Eruca sativa Mill.). Journal of Advanced Research, 19, 39–48.